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KMID : 0357920060400010009
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Korean Journal of Pathology
2006 Volume.40 No. 1 p.9 ~ p.16
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Expression of the 14-3-3 sigma Protein and Methylation Status of the 14-3-3 sigma gene in Biliary Neoplasms
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Song Dong-Eun
Yu Eun-Sil
Shim Yhong-Hee
Lee Seung-Gyu
Park Hae-Joung
Lee Young-Joo
Kim Myung-Hwan
Lee Sang-Soo
Kim Sun-Jung
Jang Jin-Se
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Abstract
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Background: The 14-3-3 sigma ( ) protein has a negative regulatory role in the cell cycle progression of the. Down-regulation or overexpression of the 14-3-3 protein has been reported in various human cancers.
Methods: Immunohistochemistry for the 14-3-3 protein was performed in non-neoplastic bile duct cells, intraductal papillary neoplasms of the liver (IPNL), mass-forming intrahepatic cholangiocarcinomas (ICC) and non-papillary extrahepatic cholangiocarcinomas (ECC). We investigated the methylation status of the 14-3-3 gene in 45 cases of these 3 tumor groups.
Results: The non-neoplastic bile duct cells demonstrated negative or weakly positive cytoplasmic immunoreactivity for the 14-3-3 protein and no methylation of the 14-3-3 gene. Overexpression as well as negative immunoreactivity associated with hypermethylation of the 14-3-3 protein was observed in 16 (69.6%) of 23 cases of IPNL, in 21 (63.6%) of 33 cases of mass-forming ICC and in 27 (71.1%) of 38 cases of non-papillary ECC. Negative immunoreactivity was increased in the invasive IPNL (4/6, 66.7%), as well as in the poorly differentiated cases of mass-forming ICC (8/12, 66.7%) and the non-papillary ECC (5/8, 62.5%).
Conclusions: The similar rates for the abnormal expression of the 14-3-3 protein among the three groups of biliary neoplasms indicate its general association with biliary carcinogenesis. Furthermore, the loss of the 14-3-3 protein may be involved in the tumor progression and differentiation in the biliary carcinogenesis.
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KEYWORD
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14-3-3 proteins, Bile ducts, Biliary tract neoplasms, Immunohistochemistry
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